And the silence of the shining stone
like the shattering crystal of the poet cries:
"I the enigma undefined,
the error of my age;
the players and the play defamed
that tread a timeless stage.
Ay! both hero and the rake,
of tragedy and of comic parts;
the dirge at many a wake
and the wit that veins the farce."
Oscar Wilde
This layout is an Act of Clawed.
Friday, June 29, 2001
this makes one more that came out streaked. Bill, my overseer, is helping me this time, so hopefully it will be he who screws up and not me.
I will be leaving for CV tonight and will not be back until Monday probably.
Ok- back to my almost-sane state. The last post was made in the dead of night, (Must take an aside here to point out the suddenly-noticed phrasing of that term- Bird,does this "The Dead of Night" make you think of anyone? Just curious.) while in a sugar-overload that had just been killed by a few letters from NAU and various other sources. But everything's straightened out and it's all good. Still going to kill PCR machine- what is supposed to come out as a nice bright band across the gel came out as a nice bright set of streaks. But that's another story. Went up and watched Stargate and Vampire Hunter D and a whole slew (betcha rarely see that word used with Anime!) of interesting Anime videos with a friend, of which I'm going to find, now that I've seen it twice, Goin' Postal - Limp Bizkit - Break stuff. Fit my mood.
Ok- now I really am going to kill the PCR stuff! Not the machine, the whole damn setup! AAAAAAAAAAAAAAAAAAA!!!!!!!. I mean, First the buffer screws up the gel- and the DNA was all wrong. Then the PCR machine screws up, and leaves my second set of DNA out at room temperature all afternoon. Today, Just When I Finally Got Things Worked Out, I Poured A Gel To Run Them On and When I Went To Load The DNA, THE FRELLING THING RIPPED!!! Something in that lab is out to get me....
Shadow: pats her arm- feel better? Clawed: grabs cold Dr. Pepper -drink this, GM. Suger makes everything better. GameMaster: glares at her henchmen - if you two aren't going to do anything useful, like go blow the place up, then get out of here! Shadow: Do you think she means it? Can we really have our way with it? Clawed: It's worth a shot!
GM: thinks about Bird - how do you put up with all of yours?
EEEEEEEEEEEEEEEEE! ::giggle:: I just finished my game and ohhhhhhh is it fun!! (No, Bird, not that game, Myst 3) eeee! And I have a brand-new (well, in actuality, reeealy old) Ellery Queen novel to ocupy my brain with!!! ::sighs blissfully at the window:: I am going to curl up on my bed with a cold, sweet white - flesh extra- ripe peach and vanish into my book for several hours- my roommate just vanished into the shower! ::purrs::
Gotta love sunburns- Yes, they almost are an instantaneous way to tan, but two weeks and two gallons of Aloe Vera later, I am still peeling. 9.9 It's a wonderful thing.
Yeah! I love Pell Grants! No More Roommate- for the year, anyway, unfortunately I'm stuck with the one I've already got.
Inadvertantly, a young teenage boy, who shall remain nameless, has discovered a really *good* way to draw the attention of both Clawed and Shadow and have them contemplate working together. At a dance friday night, he decided not only to -ahem- "verbally abuse" several girls, he thought it appraprpriate to - ahem - "grab" Sam. Big Sister carries a knife- literally- and the next time she sees Mr. Overactive Hormones, she thinks she just might let Clawed and/or Shadow take out a few of their frustrations on him. Ok, that's my rant for the day.
Other than that, the one thing on everyone's mind is the rain- or lack of it. Oh, it's rained- little pissy rain that threatens violence, pees on us for about ten minutes, then turns back into clouds. Where's a good thunderstorm when you need it?
And I really am going to start bashing PCR equipment. I had three primer sets (if you don't know what they are, they're annoying things invented to drive me nuts) to run out on a gel (see definition of primers), which is actually a fairly easy task. Except the buffer in the gel rig (that thing on which one runs gels) decided to screw up the gel, and therefore my primers. Ok- so I'll run them again- of course, I now have to start over... so I mix up the Master Mix, load it into the PCR machine, and leave for the day- SOP. This is at 1:45.
5:00. I get a phone call from Joe, our Lab coordinator. He tells me that he moved my primers from one machine to the other. CaS: why? Joe: becaue the other machine gave an error message. Don't worry- I've seen it before- CaS: (thinks) then why didn't you fix the machine? Joe: and it's nothing you did. Unfortuantely, it seems to have shut off after only about five minutes. CaS: which means...? Joe: When sis you put them in? CaS: two Joe: uh-oh. they've been sitting at room temperature all day then, haven't they?
You ever heard the thing that goes: "I'm sorry, sir, your dog died." "how did he die?" "When your house burned down." How did my house burn down?" "When the candles at your father's funeral tipped over." Apply that to DNA and you've got me banging my head against the wall and contemplating taking a sledgehammer to various PCR machines...